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Cells were washed twice and changed into Seahorse XF DMEM or RPMI medium (Agilent Technologies, 103680-100, 103681-100) containing 10 mM glucose, 0.5 mM pyruvate, 2 mM glutamine adjusted to pH 7.4

Cut-off values have been established for these biomarkers, but although they may help physicians identify the most appropriate time to start treatment for kidney damage, no corresponding treatment exists (84)

The potential of optimising host inflammatory responses by modifying Se dietary intake has been explored in several inflammatory-based disease models such as cancer ( Reference Banning, Florian and Deubel 5 ) , CVD ( Reference Flores-Mateo, Navas-Acien and Pastor-Barriuso 6 ) , mastitis ( Reference Aitken, Karcher and Rezamand 7 ) and osteoporosis ( Reference Cheng, Stabler and Bolognesi 8 )