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Secondary antibodies were HRP-conjugated goat anti-rabbit IgG (1:5,000 dilution, Thermo Fisher Scientific)

These post-injection guidelines are crucial in ensuring optimal healing and preventing any complications

Decreased 2,3-DPG, operating as a regulator of the oxygen affinity of RBC (Duhm, 1971) reduces the ability of RBC to release oxygen, resulting in tissue hypoxia (MacDonald, 1977