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The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

Future research should focus on clinical trials to establish efficacy, improve bioavailability, and create standardized formulations of OSCs for cardiovascular disease prevention and management

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[15] The last of the 20 common amino acids to be discovered was threonine in 1935 by William Cumming Rose, who also determined the essential amino acids and established the minimum daily requirements of all amino acids for optimal growth