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At present, the most commonly used methods are gene-modified bacteria and surface-modified bacteria, and some natural bacteria such as Lactobacillus (LAB) and Escherichia coli Nissle1917 (EcN) had been proved to be safe clinically (49, 50)

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Clemmons DR, Moses AC, McKay MJ et al (2000) The combination of insulin-like growth factor I and insulin-like growth factor-binding protein-3 reduces insulin requirements in insulin-dependent type 1 diabetes: evidence for in vivo biological activity

While GH therapy modulates markers of bone resorption and formation, its effects in patients with idiopathic osteoporosis are disappointing, with oestrogen therapy or bisphosphonates proving to be more effective in post-menopausal women