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23 Ferroptosis was recently linked to various chemical-induced forms of liver injury such as alcoholic liver disease, 78 methionine/choline-deficientinduced non-alcoholic steatohepatitis (NASH), 79 and arsenic-induced NASH

[7] [29] [33] During a clinical trial on the effectiveness of riboflavin for treating the frequency and severity of migraines, subjects were given up to 400 mg of riboflavin orally per day for periods of 312 months

Mitochondrial ROS were stained with MitoTracker Red CM-H 2 X (Invitrogen) as described in 71