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The pK a for an amino acid is an acid dissociation constant that refers to the equilibrium between the protonated and deprotonated forms of the backbone amino group, backbone carboxyl group, and any potential acid/base component of the variable group

Further, a new RNA-guided CRISPR activation (CRISPR-Act3.0) system has been introduced in plants for simultaneous editing of multiple genes based on the deactivated Streptococcus pyogenes Cas9 (dSpCas9) and demonstrated in tomato (Pan et al., 2021)

Uribarri J, Oh MS, Carroll HJ