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To determine the diastereomeric ratio values, the sample was analyzed by chiral analytical HPLC with a CHIRALPAK IA-3 column (150 4.6 mm, 3 m) at room temperature (flow rate 1 ml min 1 , 40% acetonitrile in H 2 O with 0.1% trifluoroacetic acid)

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10.3389/fneur.2017.00397 27

Because of the timing of the IM-MS development, this technique did not contribute to the discovery of the protein intrinsic disorder phenomenon and was not used in early studies on IDPs