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Description

UpToDate (updated as of 2025).

The curve is symmetrical around the mean, with the highest point at the mean

After washing and quenching, slides were blocked with buffer (1% bovine serum albumin (BSA) and 2.5% normal goat serum in TBS-T) for 20 min

Crude cell extracts are prepared by lysing cells, which to date has been achieved using homogenization, 127130 sonication, 125,131 bead-beating, 131,132 or enzymatic lysis