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Description

Yes when performed in a clinical setting under the supervision of licensed nurses or physicians

Anti-FLAG magnetic agarose beads (Pierce) were washed on a magnet with 1 mL of ice-cooled IP buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM MgCl 2 ) twice and resuspended to their original volume in IP buffer