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Cytotoxicity evaluation using viability assay For antitumor assays, the tumor cell lines were suspended in the medium at concentration 5 10 4 cell/well in Corning 96-well tissue culture plates and then incubated for 24 h

IV administration can occasionally cause mild nausea, and rapid infusion rates (over 1,000 mg in under 10 minutes) can trigger vasodilation and lightheadedness

Gen Comp Endocrinol 258:119133 Zeleznik AJ, Saxena D, Little-Ihrig L (2003) Protein kinase B is obligatory for follicle-stimulating hormone-induced granulosa cell differentiation

Also, eat lots of leafy greens, asparagus, beets, and mushrooms