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Among them, eight classes are distinguished: alpha (), mu (), sigma (), omega (), pi (), theta (), zeta () and kappa ()

Afterwards, the mixtures were centrifuged at 2,500 rpm at 4C for 10 min and 20 l of the supernatant that contained the -GC product was added to a half-area black 96-well plate (Corning, 3904)

This way, O 3 T produces a controlled and transient oxidative stress that indirectly will induce an adaptive response of the organism, with overregulation of Nrf2 (which leads to potentiation of antioxidant systems) and downregulation of NF-k and proinflammatory cytokines (Re et al., 2014

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