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Membranes were blocked in 1X TBS-T (Tris-buffered saline with 0.1% Triton X-100) with 5% BSA (sterile filtered) for 1 hour at room temperature, incubated for 1 hour with anti-4-hydroxynonenal (HNE) antibody (Abcam #ab46545, diluted 1:1000) and anti-actin antibody (Santa Cruz Biotechnology #sc-8432, diluted 1:500), washed and then incubated with the appropriate IRDye secondary antibodies (LI-COR)

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