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The sections were then incubated with primary antibody against 4-HNE (ab46545, Abcam, 1:250) or XBP1 (ab220783, Abcam, 1:100) at 4 C overnight, then secondary antibody (For 4-HNE: Peroxidase AffiniPure Goat Anti-Rabbit IgG, 111035144, Jackson ImmunoResearch

It appears to mimic the actions of thrombomodulin, capable of binding to thrombin and neutralizing its effects

The increased expression of ChaC2 is associated with the high expression of p53 (50), and ChaC2 is the upstream regulator of the main antioxidant regulator Nrf2 (78)