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Subsequently, the tissues were transferred sequentially into 10, 20, and 30% sucrose solutions in phosphate-buffered saline (PBS) at 4C until they settled

We have taken advantage of the ease with which ligand complexes can be produced in GR crystals, a property that extends to the isomorphous GRTR crystals, and have produced and analyzed crystals of GRTR complexes with glutathione, trypanothione, glutathionylspermidine and of a true catalytic intermediate, the mixed disulfide between trypanothione and the enzyme

Intranasal Selank requires no such preliminary steps and is ready to use as is