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Accessory proteins such as zonula occludens (ZO)-1, ZO-2, AF-6, 7H6, and cingulin function as essential molecular bridges connecting transmembrane tight junction proteins to the intracellular actin cytoskeleton, thereby playing a critical role in preserving the structural and functional integrity of tight junctions (148)

2.4 Biosynthetic gene clusters and secondary metabolites prediction FASTA-formatted contigs were subjected to analysis with BAGEL4 to identify BGCs involved in the production of antimicrobial peptides, including bacteriocins ( 2.5 Pangenome analysis Pangenome analysis was performed using Roary (v1.007001) 2 (Page et al., 2015), with MAFFT v7.427 ( Lactobacillus ( n = 9) were retrieved from NCBI (Supplementary Table 4) selected based on their origin from plant- or fruit-derived (vegetal) substrates and the availability of high-quality assemblies with complete metadata, to ensure ecological relevance and robust comparative analyses

7 , e1002145 (2011)

10.1042/cs20160604 75 Santos-CancelM.SimpsonL