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Timeline of changes after stopping GHK-Cu The post-discontinuation timeline follows predictable patterns based on skin biology and collagen turnover kinetics, though individual variation creates significant range in experiences depending on treatment duration, application frequency, concentration used, baseline skin condition, age, lifestyle factors, and genetic collagen production capacity

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To further verify whether OGD treatment could cause endothelial cell senescence, -galactosidase (SA--gal) staining was performed on the endothelial cells treated with OGD