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The classical metastatic cascade starts from a primary, epithelial, neoplastic lesion and includes: (1) epithelial mesenchymal transition (EMT) with a breach of the basement membrane barrier

Clinical observations suggest this combination accelerates results by 30-50% compared to either therapy alone

Research has shown that GHK Cu: Downregulates TGF-beta1 (the primary pro-fibrotic cytokine) in fibrotic cell models Upregulates decorin a proteoglycan that inhibits TGF-beta1 signalling and organises collagen fibril structure Reduces expression of MMP inhibitors, allowing physiological matrix remodelling to proceed This dual action promoting organised collagen synthesis in healing wounds while suppressing dysregulated fibrotic collagen makes GHK Cu of interest in research models of skin fibrosis, liver fibrosis, and lung fibrosis

Subsequently, the tissues were cut open along one side and laid flat on glass slides