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80 These protein complexes play an important role in fibroblast activation in the kidney, and their inhibition reduces or prevents the progression of CKD

While many of those results may be attributed to GSH depletion, the contribution of GCLC to -glutamyl peptide synthesis and glutamate scavenging may also play a very important role, particularly in the context of xCT inhibition, where cells cannot export glutamate

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The samples were subsequently incubated with 10 M mitoSOX working solution at 37 C for 30 min under light-protected conditions, followed by nuclear staining with Hoechst 33,342 solution for 5 min