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This is a special type of affinity chromatography called Immobilized Metal Affinity Chromatography (or IMAC) , in which nickel is covalently coupled to agarose using the chemical compound nitrilotriacetic acid (NTA)

The only exceptions from these rules relate to recommended large doses of stable (non-radioactive) iodine either to protect the thyroids of a given population exposed to radioiodine during a nuclear power plant accident [121] or in certain diagnostic and treatment procedures that are necessary for some individuals