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Dephosphorylated RNAs were then immediately radiolabelled using T4 PNK and [- 32 P] ATP in rCutSmart buffer (NEB, B6004S) supplemented with 5 mM DTT (Invitrogen, 15508013) at 37 C for 12 h, then desalted using Micro Bio-Spin P-30 columns (Bio-Rad, 7326250), Micro Bio-Spin P-6 columns or Amersham MicroSpin G-25 columns (Cytiva, 27532501) and gel-purified on a urea-polyacrylamide gel

ROS directly cause lung damage as a result of DNA, lipid, carbohydrate, and protein alterations, and activate local inflammatory responses that contribute to COPD development and progression (7983)

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Mazza MG, De Lorenzo R, Conte C, Poletti S, Vai B, Bollettini I, Melloni EMT, Furlan R, Ciceri F, Rovere-Querini P, Benedetti F (2020) Anxiety and depression in COVID-19 survivors: role of inflammatory and clinical predictors