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2.3 Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)

For specialized applications, a nebulizer operating around ~16 psi (max ~32 psi 5 psi) is commonly referenced

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This correlates with the quality assurance unit's insufficient adoption of responsibility for this area, as described in 211.22, which is also the most frequently cited GMP deficiency in this fiscal year