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The column temperature was maintained at 40 C, and the flow rate was set to 0.8 mL/min

Simplistically, non-enzymatic oxidation leads mainly to metabolites in which the sterol ring system is oxidized, while enzymatic processes usually yield oxidized side chains (7hydroxylation is an exception)

Specifically, 2-AG is able to induce channel block and inhibit Na + currents or induce changes in biophysical properties of activation/inactivation of Na v channels, thereby affecting their function [68] (Fig