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The retrospective, single-arm design without a control group limits causal inference, as placebo effects, regression to the mean, natural symptom fluctuation, and the standardized rehabilitation protocol may have contributed to observed improvements

& Kihara, A

GLUTATHIONE An amino acid that contributes to immune defense because it is required by the immune system for two important reasons: it protects host immune cells through its antioxidant mechanism and it provides the optimal functioning of lymphocytes and other cells of the immune system

Subsequently, 25 l of either the total glutathione lysis reagent or the oxidized glutathione lysis reagent was added to each well and the plate was shaken on an orbital shaker for 5 min