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And so a lot of the joint pain youre feeling and a lot of like the, uh, nerve neuropathy and the whole, like, thats all just a reaction to your body

Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific 15-340-154) containing lysis buffer (Tris-HCl 100 mM, 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor Cocktail and homogenized using a bead mill (VWR) for 10 seconds

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PNM-10 showed a statistically significant increase in axon number at the distal end as compared to the conduit-only group (5962 1193 vs 1903 1194, p 10 mm owing to a lack of vascular support and nutrient/waste exchange 32