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Cancer Cell, 21 , 8291

Human cells Normal Human dermal fibroblasts (ATCC, PCS-201-012) were grown in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% (v/v) fetal bovine serum, 10 mM glutamine, pH 7.4

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The sequence of reactions may be formulated as follows: (6) Enz-[Por-Fe(III)] + nH 2 O 2 = Enz-[Por+.-Fe(IV)=O] + H 2 O + (n-1)H 2 O 2 (7) Enz-[Por+.-Fe(IV)=O] + (n-1)H 2 O 2 = Enz-[Por-Fe(III)] + H 2 O + O 2 + (n-2)H 2 O + (n-2/2)O 2 Adding Equations 6 and 7 gives: (8) nH 2 O 2 = 2H 2 O + O 2 + (n-2)H 2 O + (n-2/2)O 2 Which simplifies to: (9) nH 2 O 2 = nH 2 O + n/2 O 2 Finally, doubling both sides of Equation 9 and dividing through by n, gives Equation 1: (1) 2H 2 O 2 = 2H 2 O + O 2 By these reactions effectively reducing back to Equation 1, it implies this preliminary network hypothesis presented here does not contradict the known catalase H 2 O 2 disproportionation chemistry that has previously been discovered and investigated