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For those with positive GPX4 expression, we categorized GPX4 + if 5% to 24% of cells were positive, GPX4 ++ if 25% to 75% of cells were positive, and GPX4 +++ if more than 75% of cells were positive after IHC staining

(967) developed a mass spectrometric method to measure nitrotyrosine of smokers, but was surprised to find significant amounts in the urine of non-smoking humans as well