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Description

Antibody-stained protein signal was amplified and visualized using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher #34578) and imaged with a ChemiDoc MP Imaging system (Bio-Rad)

To calculate absolute (i.e., water-referenced) GSH levels from GSH/Cr ratios, concentrations of total Cr (Cr plus phosphocreatine) were calculated

Through robust computational algorithms with self-checking (causal vs anti-causal estimates) and auto-correcting (pole-zero cancellations), this powerful signal processor gives ultimately the most accurate molecular content of the MRS-scanned tissue