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doi: 10.1016/j.procbio.2019.10.027 165 YonathanK.MannR.MahbubK

The reaction mixture was incubated at 37 C for 1 h, and then 1 mL of thiobarbituric acid (TBA) solution (1%, w/v) and 1 mL of trichloroacetic acid (TCA) solution (2.8%, w/v) were added to the mixture and heated in a boiling water bath for 15 min