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All samples were collected with the patients written informed consent and approval from the Sixth Affiliated Hospital of Sun Yat-sen University Review Board (ethics code: 2021ZSLYEC-100)

4.3.2 In vivo studies Treatment of acute lung injury induced by LPS in mice with nanoemulsions containing pequi oil-oleic acid (20 mg/kg), reduced myeloperoxidase (MPO), TNF-, IL-1, IL-6, MCP-1, and KC levels and pulmonary leukocyte accumulation if administered orally, which can be a substitute strategy to treat respiratory inflammatory disorders (De S Coutinho et al., 2020s)

Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature

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