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All reagents and experiments conducted in this study are compliant with the institutional biological safety committee (SPROTO202200000081) of the University of Arkansas for Medical Sciences

DNA methylation typically occurs at CpG sites, where cytosine in the CpG dinucleotide environment is methylated at the fifth carbon position by DNA methyltransferases (DNMTs), resulting in 5-methylcytosine (5 mC) (Figure 1)

The lips have thin, delicate skin and limited melanin protection

The combined effects of these mechanisms provide survival advantages to tumor cells, promoting tumor progression, treatment resistance, and immune evasion